Next Generation Sequencing: Amplicon-EZ
Amplicon sequencing made easy!
- Easy on your budget – as low as €53/£47 per sample
- Easy to order – checkout online in minutes
- Easy to submit samples – use a GENEWIZ drop box
- Easy to analyze data – interactive reports with intuitive design
- Easy on your deadline – results in 8 business days*
Amplicon-EZ is a streamlined version of next generation sequencing to analyze heterogeneous PCR products. Our standardized workflow simplifies ordering, processing, and analysis to deliver high-quality results fast. Applications include CRISPR screening, variant genotyping, antibody discovery, and much more.
To make our Amplicon-EZ service even easier and quicker for European customers, processing is now available at our European Genomics Headquarters in Leipzig, Germany.
Amplicon Sequencing in 8 Days*
1. Sample submission
- No minimum sample number
- Convenient sample submission via dropboxes
2. Library Preparation
- 150-500 bp products accepted
- Submit with or without lllumina® partial adapters
3. Sequencing
- -50,000 reads per sample
- Full sequence coverage with 2x2S0 bp sequencing
4. Data Analysis
- Interactive variant report
- Unique sequence identification and abundance analysis
*Samples are processed at our European Genomics Headquarters in Leipzig, Germany in cycles starting on Wednesdays with data returned 8 business days later (on the second Monday).
Applications: CRISPR SCREENING & MORE
CRISPR Screening with Amplicon-EZ
1. Edit cells with CRISPR
2. Amplify target region from edited cell populations
3. Use Amplicon-EZ to expedite sequencing of PCR products
4. Analyze results to find wells with desired mutations**
CRISPR Validation with Amplicon-EZ
1. Isolate clones from postitive wells (see CRISPR screening)
2. Amplify target region from clones
3. Use Amplicon-EZ to expedite sequencing of PCR products
4. Validate edits and find homozygous mutants**
**View our interactive report (login required)
Want GENEWIZ to perform amplification and sequencing? Learn about our additional NGS-based CRISPR validation services.
NGS PLATFORMS
For information on our NGS platforms as well as recommended configurations of your projects, please visit the NGS Platforms page. GENEWIZ does not guarantee data output or quality for sequencing-only projects.
